Dr. Tan and Partners is one of the few approved Anonymous HIV Testing Centres in Singapore. A detailed write up on our service is available here.

Other STD services: Early STD Screening, Rapid Hepatitis B Blood Test, Swab for Gonorrhea and many more... contact us to make an appointment now!

HIV P24 Ag/Ab Test, Duo Test, Combo Test at our Clinic

Updated information on Rapid Anonymous HIV Combo test at our Clinics. 

 

Info leaflet for SD Bioline Combo Test

Info leaflet for SD Bioline Combo Test

I have been getting a lot of question with regards to the Duo test or Combo test as it is also known as.

Let’s first get some basics right:

Duo Test = Combo Test = P24 Ag/Ab test

All these terms refer to the same thing. Basically it is a test that simultaneously tests for the HIV P24 Antigen and the HIV antibody.

HIV Prevention within 72 hours of exposure (PEP) – Click Here

The HIV P24 antigen is a small piece of protein that is found on the capsule of the HIV virus. When a person is infected with HIV, these bits of protein can be found floating in the blood. The HIV P24 test is a test that detects these bits of protein. This test was first developed as a HIV screening test but rapidly ran out of favor due to the development of more advanced NAAT tests. (For more details on NAAT testing please read: http://www.drtanandpartners.com/nucleic-acid-amplification-testing-naat/)The window period for P24 testing is also very small. This test alone is only accurate for between 3 and 6 weeks post exposure. So it is a test with very limited applications unless combined with the HIV antibody test. The presence of P24 Antigens in the blood indicates a recent HIV infection.

Click here for details on getting the Combo test at our Clinics

We all already know that the HIV antibody test becomes quite reliable at 4 weeks and as good as conclusive at 6 weeks. Although most health authorities still recommend a 3 months window period. So putting these 2 tests together gives you a very powerful tool to diagnose HIV infection in as little as 3 weeks. Personally I prefer to conduct the Combo test at 4 weeks because I believe that is when the level of P24 antigen peaks in the blood giving us maximum accuracy.

In our clinic, we use the SD Bioline Rapid HIV Combo Test 2.0. It is a rapid test that takes only 20 minutes for us to get results. We are also approved to conduct this test as part of the Anonymous HIV testing Program.

The test is conducted very much like the HIV antibody test. All we need is blood from a finger prick. (For more details on Anonymous HIV Testing at our clinic please read: http://www.drtanandpartners.com/anonymous-hiv-testing-at-our-clinic/)

SD Bioline HIV Combo Test showing a positive result (2 red bars)

SD Bioline HIV Combo Test showing a positive result (2 red bars)

 

 

 

 

 

 

 

 

 

SD Bioline Combo HIV test showing a positive result. Note the test line is very faint. This could be due to a very recent HIV infection or the test could be false positive (1% chance).

SD Bioline Combo HIV test showing a positive result. Note the test line is very faint. This could be due to a very recent HIV infection or the test could be false positive (1% chance).

 

 

 

 

 

 

 

 

 

Multicentre clinical trials have shown 100% sensitivity and more than 99% specificity.

This kit has been registered with Singapore’s Health Science Authority (HSA).

So the Combo Test has many advantages including:

1. Earlier detection of HIV infection.

2. Rapid (20 minutes)

3. Anonymous

At our clinic the Combo Test costs $150.

Click on the picture for the latest information on the HIV testing Window Period

Click on the picture for the latest information on the HIV testing Window Period

 

 

 

 

 

 

 

 

 

Click here for HIV Testing FAQ

More on HIV symptoms.

If you have any questions, visit our free online forum on sexual health, HIV and STDs.

Click here for Info on STD Symptoms in Men
Click here for Info on STD Symptoms in Women
Click here to see the full range of our HIV/STD Services

If you need a HIV test visit Our Clinics anytime during our opening hours. You do not need an appointment.

We are now open on weekends.

Find out more about Anonymous HIV testing at our clinics.

Can’t wait for 3 months to find out? Find out more about Anonymous Rapid HIV Combo test at our clinics.

If you had a high risk exposure to HIV within the past 72 hours, you can take medicines to reduce your risk of actually contracting HIV. Find out more on HIV PEP Treatment.

We have 4 locations conveniently located at Robertson Walk, Bencoolen Street, Novena and Scotts (Pacific Plaza).

Robertson Walk            6238 7810

Bencoolen Street          6884 4119

Novena                           63972095

Scotts                              66942348 (Opening April 2015)

 

We are Open on Saturdays and Sundays.

Click Here for Contact Details, Address and Opening Times.

Related Topics:

Anonymous HIV testing at our Clinics

HIV – Understanding the Window Period

Cost of HIV/STD tests at our Clinics

Condom Broke

Getting HIV from Oral Sex

More on HIV symptoms

How long can HIV live outside the body?

 

About Dr. Tan
Dr. Tan graduated from the National University of Singapore in 2001. His residency was in the two largest public hospitals in Singapore; Tan Tock Seng Hospital and Singapore General Hospital.

Find the profiles of our panel of doctors.

Consultation $20.00-80.00
Rapid test only $20.00
(not inclusive of test)
8am – 5pm $60.00
5pm – 9pm $80.00
STD screening
Standard HIV, Syphilis, Hepatitis B/C, Herpes Simplex Virus Type I and II $ 200.00
Male
Urethritis
Urine – Chlamydia and Gonorrhoea PCR, Monilia, Trichomonas, NSU, Mycoplasma, Ureaplasma, Urine Culture, Candida $ 280.00
Female
Urethritis and Vaginitis
Vaginal Swab – Chlamydia and Gonorrhoea PCR, Monilia, Trichomonas PCR, Gardnerella PCR, Candida PCR, Group B StrepNSU, Mycoplasma, Ureaplasma, Direct Microscopy, Candida, Vaginal swab culture $ 345.00
Comprehensive (Male) Blood – Syphilis, Hepatitis B/C, Herpes Simplex Virus Type I and IIUrine – Chlamydia and Gonorrhoea PCR, Monilia, Trichomonas, NSU, Mycoplasma, Ureaplasma, Urine Culture, Candida $ 390.00
Comprehensive (Female) Blood – Syphilis, Hepatitis B/C, Herpes Simplex Virus Type I and IIVaginal Swab – Chlamydia and Gonorrhoea PCR, Monilia, Trichomonas PCR, Gardnerella PCR, Candida PCR, Group B StrepNSU, Mycoplasma, Ureaplasma, Direct Microscopy, Candida, Vaginal swab culture $ 525.00
Individual STD Tests
Determine Syphilis test Rapid Syphilis Test $ 50.00
Determine Hepatitis B Test Rapid Hepatitis B Test $ 50.00
Herpes Simplex Virus PCR Herpes Simplex Virus Polymerase Chain Reaction (PCR) Test $ 190.00
Chlamydia and Gonoccocal PCR Chlamydia and Gonorrhea  $188.00
Herpes Simplex Virus Type I IgG Antibody Herpes Type 1 $ 40.00
Herpes Simplex Virus Type II IgG Antibody Herpes Type 2 $ 40.00
Non-Specific Urethritis (NSU) Culture Mycoplasma, Ureaplasma $ 118.00
HIV test
HIV RNA/DNA Polymerase Chain Reaction (PCR) Test $600
Add $100 for expedited results
Rapid Fingerprick Blood HIV-1/2 P24 Antigen/Antibody Combo (HIV Duo Test) (4th generation!) $ 150.00
Rapid Oral Saliva HIV-1/2 Antibody Test (Oraquick) $ 50.00
Rapid Fingerprick Blood HIV-1/2 Antibody Test (Determine) $ 50.00
PEP $2000 – $2200

1,163 Comments

  1. Hi Dr. Tan,
    I have unprotected sex with a CSW in Bangkok. After 3 weeks post exposure, I have done the HIV I/II Ag/Ab test in Malaysia Pathlab. The result is non-reactive.

    Thus on the 4th week, I had experienced urine pain but I did not do any further test. On the 6th week, a doctor has taken my blood sample to test for all STDs, including HIV I/II Ag/Ab test by Gribbles. The result for HIV is again non-reactive. However, the test also showed that I had Chlamydia. I had to take 2 courses of Zitromax.

    It is 9 weeks and now I have mild fever and diarrhea for the past 2 days.

    I wish to wait for 12 weeks to perform another HIV test but I am worried now if the 3rd week and 6th weeks test are conclusive? Is the Malaysia lab using the same test standard as Singapore?

    I can only be back to Singapore on the 12th week and do a test in your clinic.

    Please advise should I test in Malaysia again now?

    BR
    Saji

    • Dr Justin Sim

      I cannot say for sure if Malaysia uses the same standard of tests. What you can do now is get the 4th generation rapid combo test done as this will be conclusive for your timeframe. If you wait until you can see us at 12 weeks we can still do the same test.

  2. dear Dr. I got tested at DBS /AFA/ by 4th generation test. The kit seems same one as your clinic uses. But when I saw the kit there were two res lines, i dont exactly remember, I think one was on C, the other one was in above the C. The dr or consultant said the result is negative and showed me the kit. But when I asked what is the one red line on the kit, she said this is for test complete sign. I just belive in her. but when I checked the internet its stated that there must be only one res line to show negative. Am I right? Can it be possible there are two redlines whcih stand for Complete and Control? Thank u Dr

    • I am not going to second guess the doctor you saw. If she says it was negative, then I do not see any reason to not believe her. As far as I know, a negative test has 1 red line at C which stands for control. If you have any further queries I suggest you bring it up to AFA.

  3. Hello Dr. Tan

    i tested on 4 weeks mark using HIB Combi PT test by ROCHE.

    is this conclusive?

    thanks alot for ur time.

  4. Hi Dr tan,
    I had a risky exposure (anal i was receptive) where I acquired gonorrhea. 30 days after exposure I had a 4th generation Elisa p24 antigen test that came out negative, this was in the USA. Also I had no typical HIV ads symptoms, just gonorrhea symptoms.
    Can I take this as conclusive and if I tested at your lab would you recommend further testing?

  5. Dear Dr. Tan,

    I have the following results:
    Alere Combo Day 10: Faint positive obtained.
    SD Bioline Combo Day 28: Negative obtained.
    SD Bioline Combo Day 35: Negative obtained.
    Alere Combo Day 62: Faint positive obtained.
    AHP Test Kit Day 62: Negative obtained.

    Why is the result always changing, as it is always faint positive for the Alere Combo Rapid Test Kit but all negative for the other test kit?

    Any advice on this? I am very confuse.

    • Dear Dr. Tan,

      I am worried, can you help me on this?

      • i recommend you take eclia combo if you are within six weeks to three months , ifree you are beyond three months you can take eclia , a combo test looks for antibody as well as antigen , the more reliable way is to take the antibody test after three months , i hope you were not on pep , that would finalize in six months , if you are below the cut off then you dont need to test further , if you are above then you may need a western blot , keep in mind that western blot can come up inconclusive in healthy individuals who are not infected , stop at whatever stage you are negative , if positive or indeterminate then rna pcr is the final test which tells whether the attack is under control or the it is going towards a disease , if god forbid a pcr is positive then the only solution is medication as advised by the doctor if you are willing to take it

  6. If a person is infected HIV2 it would stay within the range of the 28 days ? . Studies on this? The cdc only talks about igm for HIV but is not clear about the HIV2

    • Very limited studies on HIV 2. Personally if I suspect HIV 2, I would test up to 3 months.

      • Dr, but you elsewhere in this forum said igm the vh2 are collected not later than the 25th then what is telling me is contradictory.

  7. Hello doctor, does the hiv duo second window period exist, where the ag/ab are both below the threshold, thus providing false negatives? You said the test is conclusive at 28 days, does it have to be exactly 28 days or is more than 28 days also conclusive ( don’t mind if this sounds stupid).
    Thanks

    • I have actually answered this question a few times before. You are asking if there is a cross over point where both the antigens and antibodies are too low for detection. As far as I know, this does not happen. The combo test is conclusive at 28 days and anytime thereafter.

  8. Sebastian r

    Dear dr tan, The test detects combo igm maximum at 24 days as you and I’ll explain the question is this includes hiv 2?

    • Sebastian r

      Dr please is for my hope

      • ab ag combo targets hiv 1 ag at three weeks and both hiv 1 and 2 ab at six weeks , if the result is negative and the number is not too close to cut off then you dont have hiv , in case of pep you can only test at three months for sure

  9. Sebastian Rpo

    Dr., Thank you for the support. I tell him that I do a test architect combo abbot to the exact 28 days and gave me no, but I wanted you to get an queries about this and to end this episode of my life;

    1.Is this kind of combo conclusive test at 28 days?
    2.I call attention to a response of this page is:
    Have there been any occasions in which you or indeed fellow colleagues of yours have found a 28 day negative turn positive?
    R/: Yes – but only with people who were clinically ill at the time, had very high risk exposures (with others who had recently become infected and so their infectiousness was very high) and so they clinically generated a high degree of concern on our part. I should say though that there are tiny numbers of these – perhaps 3 in 5 years and with the numbers of tests we do per year this is miniscule. In someone such as yourself where even if you had had vaginal unprotected sex with a female of unknown status I would regard the result as certain

    3. The question dealing with this is that if there is possibility to change the result in people suffering from cancer or other issues? I’m healthy I would have no problem?

    4. For a test to be conclusive at day 28 no matter what level of risk you have taken, what matters is that the test is negative.?

    5. This is another of the paragraph that caught my attention

    From the Freedomhealth point of view, using as described above the combined knowledge and experience of the team we regard the HIV DUO test as a conclusive HIV test at 28 days or beyond in almost all circumstances. In situations where a patient has had an exceptionally high level of HIV exposure and has symptoms which we might in our expert opinion regard as suggestive of an acute HIV infection, we will encourage the patient to re-test at an appropriate interval which we will decide with the patient. However, it is almost always the case that a negative HIV DUO test at 28 days or more will be conclusive and will not need repeating.

    6.I think this points to what you call if there is a human error that through must be another risk situation and have to repeat the test is the same as the 28 days and would be the antigen and igm?

    • Sebastian Rpo

      Dr excuse my bad English , the test gave negative and these are the questions of a product called Freedomhealth about the test at 28 days .

  10. I had protected vaginal sex with one highclass escort in India and kissing. I do not have open shores and cuts. Is there a risk of HIV or other STD .Do i need to go through tests than which tests.Please guide me.

    • I’m sorry. This forum is not for dispensing medical advice. Please see your doctor for what tests you need to do. All I can say is that you are at risk of HIV but the risk is very low. You are also at risk of other STDs.

  11. Sebastian Rpo

    Dr data to argue that combo is conclusive evidence is that IgM are in maximum blood at 24 days and p24 appears to 7 days , with this the 28th already a conclusive result . What is the reason you believe that BASH STATEMENT put a period of 8 weeks?

    • I think the recommendation to retest at 8 weeks is more to rule out human error than because of a concern of the accuracy of the test itself. Or maybe it is just for a “just in case so that we can legally cover our asses” reason. I’m not sure.

  12. Hello again the link works but the file u want to read is downloaded to your Downloads folder.For the link that u have sent to me i disearee about the holes in condoms.The holes in condoms does not exist,if they break they break obviously and u understand that u are in risk and should test.My condom has broken with my girlfriend and i have seen it how it breaks.Holes in condom does not exist and water test is in my opinion Bullshit sorry for this word.also one’s again the link : http://www.bashh.org/documents/BASHH-EAGA%20statement%20on%20HIV%20WP%20(Nov%20%2014).docx

    • Sebastian Rpo

      Here is the text for the link does not work:

      BASHH/EAGA statement on HIV window period

      November 2014

      HIV testing using the latest (fourth generation) tests is recommended in the BHIVA / BASHH / BIS UK guidelines for HIV testing (2008). These assays test for HIV antibodies and p24 antigen simultaneously. A fourth generation HIV test on a venous blood sample performed in a laboratory will detect the great majority of individuals who have been infected with HIV at 4 weeks after specific exposure.

      Patients attending for HIV testing who identify a specific risk occurring less than 4 weeks previously should not be made to wait before HIV testing as doing so may miss an opportunity to diagnose HIV infection (and in particular acute HIV infection during which a person is highly infectious). They should be offered a fourth generation laboratory HIV test and be advised to repeat it when 4 weeks have elapsed from the time of the last exposure.

      A negative result on a fourth generation test performed at 4 weeks post-exposure is highly likely to exclude HIV infection. A further test at 8 weeks post-exposure need only be considered following an event assessed as carrying a high risk of infection.

      Patients at ongoing risk of HIV infection should be advised to retest at regular intervals.

      Patients should be advised to have tests for other sexually transmitted infections in line with advice on window periods for those infections (see BASHH guidelines at: http://www.bashh.org ).

      Dr Keith Radcliffe – Chair, BASHH Clinical Effectiveness Group
      Dr Laura Waters – Chair, BASHH HIV Special Interest Group
      Prof Brian Gazzard – Chair, Expert Advisory Group on AIDS
      Dr Jan Clarke – President, British Association for Sexual Health and HIV

    • LOL! I think NIAID will be very upset you called bullshit on their study!

  13. Camilo Rpo

    Dr I value what you do for people helping them with this issue, first of all want to thank.
    Dr long before I was finding out when he did not know of your page, and I found something that I doubt genre and is a statement on BASH STATEMENT HIV window period 15th march 2010 where he said that despite the combo was very good at 28 days had to be repeated at 3 months, I wrote to a lab and they said it was because some subtypes of HIV and could not identify the so it was good to repeat at 3 months the 3rd generation, I want to ask if they have knowledge and this test has included all subtypes and me can do with ease the 28th since I live in Colombia and here make HIV architect combo Abbot?

    • Camilo Rpo

      It was already updated the text to November 2014, and says :A further test at 8 weeks post-exposure need only be considered following an event assessed as carrying a high risk of infection. That is for them a high risk referred to , that would apply in this case . if all risks are equal if they refer or if one was with hiv positive person identified , but if you do not know ?

      • I find the lab statement rather confusing. If there are subtypes of HIV that cannot be identified on tests, then why would repeating the test at 3 months make any difference? If the subtypes cannot be identified that logically they cannot be identified no matter how long you wait to take the test. I also think the recommendation to test at 8 weeks for a high risk infection is based more on the “just in case someone somewhere cocks it up” mantra rather than on the accuracy of the test itself.

  14. Sebastian Rpo

    Dr I value what you do for people helping them with this issue, first of all want to thank.
    Dr long before I was finding out when he did not know of your page, and I found something that I doubt genre and is a statement on BASH STATEMENT HIV window period 15th march 2010 where he said that despite the combo was very good at 28 days had to be repeated at 3 months, I wrote to a lab and they said it was because some subtypes of HIV and could not identify the so it was good to repeat at 3 months the 3rd generation, I want to ask if they have knowledge and this test has included all subtypes and me can do with ease the 28th since I live in Colombia and here make HIV architect combo Abbot?

    • BASH STATEMENT HIV WINDOW PERIOD has changed NOV 2014 from 12 weeks to 8 weeks ;)

      • Thank you for sharing. Can you put the reference link here please? Thanks!

        • http://www.bashh.org/documents/BASHH-EAGA%20statement%20on%20HIV%20WP%20(Nov%20%2014).docx .This is the link ;) i tested with a DUO after 10 weeks post my last exposure negative 0.05(cutoff:0.25) and i had over 2 years lot of protected oral and protected sex.Also Condoms works when use consistently

          • And HIV cannot get through Latex,no way no how.For Chris’s sake always use a condom consistently for oral and vaginal and if u don’t have any breakage or slip,u can be confident that ur test will become negative.Peace all of you

          • I completely agree that everyone should use condoms correctly and consistently. However, there have been several studies to suggest that condoms may not be absolutely 100% effective. For example this report released in 2001 (http://www.niaid.nih.gov/about/organization/dmid/documents/condomreport.pdf) on page 14 it makes the statement “Overall, Davis and Weller estimated that condoms provided an 85% reduction in HIV/AIDS transmission risk when infection rates were compared in always versus never users.” So just let me say that I agree with you in that everyone should use condoms and based on the statistics, everyone who uses condoms correctly and consistently should not be worried just like you said. However, I would like to add that even if you use condoms all the time, it would be still prudent for you to get a HIV test every so often. Again, thank you for sharing.

          • Thank you very much for sharing. Unfortunately, for some reason, the link is not working. If it is not too much trouble could you put the link up again? I really appreciate this sharing of information. We can all learn much more about HIV.

        • Anxious

          Dear Dr Tan, bashh.org -> bashh guidelines-> bashh/eaga position statement on HIV window period 14 Nov 2014. It says 8 weeks need only be considered for a high risk of carrying infection. Does it means the new confirmatory test is 8 weeks?

Trackbacks/Pingbacks

  1. HIV 4th Generation Test Singapore | HIV - […] The HIV 4th Generation Test is also called the Combo test or the Duo test. […]
  2. False Negative HIV ELISA test | HIV - […] Click here for more info on the HIV Combo test Click here for more info on the HIV PCR …
  3. Rapid Anonymous HIV Combo Test at our clinic | HIV - […] Click here to learn more about the P24 Ag/AB Combo/Duo Test […]
  4. Most Popular HIV Articles | HIV - [...] HIV P24 Ag/Ab Test, Duo Test, Combo Test at our Clinic [...]

Leave a Comment